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Tris(hydroxymethyl)aminomethane (Tris) (THAM) CAS 77-86-1

Chemical name: Tris(hydroxymethyl)aminomethane

Synonymous names:Tris;THAM;Tris Base

CAS No:77-86-1

Molecular formula:C4H11NO3

Molecular weight:121.14

EINECS No:201-064-4

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Structural formula 

Product Description

Items

Specifications

Appearance

White Crystal powder

Content    %

99.8%

Melting Point

170.0-172.0

 

Water insoluble

0.002%

Residue on ignition

0.004%

Loss on drying

0.14%

pH

10.77

Fe3+

10ppm

SO42-

10ppm

Cl-

10ppm

Heavy metal (Pb)%

10ppm

Absorbancy 40 water solution )260nm

0.60%

Absorbancy 40 water solution )280nm

0.051%

Conclusion

Qualified

 

Properties and Usage:

Tris (hydroxymethyl) aminomethane, commonly known as Tris or THAM, is a highly effective organic compound widely used in biochemistry and molecular biology. As a key buffer, Tris can ensure that the pH value of the solution remains stable in scientific experiments and industrial applications.

 

Main application areas:

 

1. Biochemistry: Tris is an ideal choice for molecular biology techniques such as nucleic acid and protein electrophoresis and polymerase chain reaction (PCR). Its buffering capacity helps researchers maintain the required pH environment in complex biochemical reactions and ensure the accuracy and repeatability of experimental results.

 

2. During the extraction and purification of DNA and RNA, the stability and buffering capacity of Tris ensure the activity and integrity of biological samples, and it is the preferred material for preparing high-quality biological samples.

 

3. As a biological buffer, Tris is a key component in the buffer system used for DNA and RNA electrophoresis, including the preparation of TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA) buffers.

 

4. Tris base, the deprotonated form of Tris, is used to treat metabolic acidosis, especially during surgery or when renal function is impaired.

 

5. Cosmetics and personal care products: In the cosmetics industry, Tris is often used as a pH regulator and antioxidant

 

6.Industrial applications: Tris has become an important material for improving production efficiency and product quality in a variety of industrial processes such as water treatment, textile processing, and plastic manufacturing due to its excellent buffering properties and low toxicity.

Storage conditions:For storage, it should be kept sealed in a dry and cool place, avoiding direct sunlight.

Packing: This product is packed in 25kg and 50kg cardboard drum, and it can also be customized according to customers' requirements

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