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HEPES CAS 7365-45-9

Chemical name: HEPES

Synonymous names:hepesfreeacid;HEPES free acid;HEPES, 1M solution

CAS No:7365-45-9

Molecular formula:C8H18N2O4S

Molecular weight:238.3

EINECS No:230-907-9

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Structural formula 

Product Description

Items

Specifications

Appearance

White crystalline powder

Content

Min 99.5%

Color (1M solution)

Tested

PKa at 20℃

7.4-7.7

Heavy metal (Pb)

Max 5 ppm

Sulfate

Max 0.05%

Ignition residue

Max 0.1%

Loss on drying (105℃, 3 hours)

Max 1%

UV absorbance/260nm

Max 0.5

UV absorbance/280nm

Max 0.5

UV absorbance/440nm

Max 0.2

Properties and Usage:

4-Hydroxyethylpiperazineethanesulfonic acid (HEPES) is an efficient biochemical buffer, mainly used to maintain the pH stability of experimental conditions, and is an indispensable tool in the laboratory.

 

1. Cell culture and biological research

HEPES is an ideal buffer in cell culture medium, which can effectively stabilize the pH of the culture medium. This property ensures that cells grow in the best environment, especially suitable for long-term and high-density culture, and helps the normal growth and reproduction of cells.

 

2. Enzymology research

In enzymology experiments, HEPES provides a stable pH environment for enzymes, optimizes their activity and stability. It is often used to prepare buffers to support enzyme functional testing and analysis, and ensure the accuracy and repeatability of experimental results.

 

3. Drug development and biopharmaceuticals

HEPES is used as a buffer in drug development and biopharmaceuticals to ensure the stability of drugs and biologics during storage and use. Its excellent buffering properties are particularly suitable for drug formulations that require long-term stability.

 

4. Immunology and molecular biology

In immunology and molecular biology research, HEPES is used to prepare a variety of experimental reagents and buffers to maintain a stable pH in the reaction system. This property improves the efficiency and reliability of the experiment and helps to deeply understand the behavior and mechanism of biomolecules.

 

5. Chemical synthesis

HEPES is used as a buffer in chemical synthesis to provide a stable pH environment, support the progress and optimization of the reaction, and ensure the efficiency of the chemical reaction and the purity of the product.

 

Storage conditions: Store tightly closed in a cool, dry place at 2-8°C.

Packing: This product is packed in 25kg 100kg cardboard drums, and it can also be customized according to customers' requirements

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